منابع مشابه
Cilia get arms for bending
Afzelius had tried to improve on existing electron microscopy (EM) contrast by preparing his samples using 40% osmium tetroxide—“a nasty mixture,” he says, “that I wouldn’t touch now.” But the chemical did its trick. Earlier EM had revealed the now familiar 9 2 pattern of the axoneme (the functional core of both eukaryotic flagella and cilia), with 9 doublet microtubules surrounding a central p...
متن کاملPrimary cilia of inv/inv mouse renal epithelial cells sense physiological fluid flow: bending of primary cilia and Ca2+ influx.
Primary cilia are hypothesized to act as a mechanical sensor to detect renal tubular fluid flow. Anomalous structure of primary cilia and/or impairment of increases in intracellular Ca2+ concentration in response to fluid flow are thought to result in renal cyst formation in conditional kif3a knockout, Tg737 and pkd1/pkd2 mutant mice. The mutant inv/inv mouse develops multiple renal cysts like ...
متن کاملStructural conformation of ciliary dynein arms and the generation of sliding forces in Tetrahymena cilia
The sliding tubule model of ciliary motion requires that active sliding of microtubules occur by cyclic cross-bridging of the dynein arms. When isolated, demembranated Tetrahymena cilia are allowed to spontaneously disintegrate in the presence of ATP, the structural conformation of the dynein arms can be clearly resolved by negative contrast electron microscopy. The arms consist of three struct...
متن کاملInteractions of dynein arms with b subfibers of Tetrahymena cilia: quantitation of the effects of magnesium and adenosine triphosphate
Tetrahymena 30S dynein was extracted with 0.5 M KCl and tested for retention of several functional properties associated wtih its in situ force-generating capacity. The dynein fraction will rebind to extracted outer doublets in the presence of Mg2+ to restore dynein arms. The arms attach at one end to the A subfiber and form bridges at the other end to the B subfiber of an adjacent doublet. Rec...
متن کاملCilia Device for Microfluid Manipulation
This paper presents bio-mimetic cilia resonated in a microfluidic device for fluid transport and bio-compatible mixing. High aspect ratio silicone cilia structures were successfully fabricated and assembled in a fluidic device by the recently developed under-water fabrication method. The cilia in a fluid were resonated by piezoinduced actuation. As an application of the cilia, a fluorophoric av...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 2005
ISSN: 1540-8140,0021-9525
DOI: 10.1083/jcb1683fta1